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MicroResp图片20210214.jpg


MicroResp是英国James Hutton公司出品,用于土壤、沉积物、水样中碳源测定的专利性装置。
在环境、土壤、水资源保护等领域应用广泛。


MicroResp™ is a unique microplate-based respiration system that enables the user to analyse up to 96 soil, sediment or water samples and test a range of carbon sources and/or replicates in a small compact space.

While some sole C-source tests tend to select for fast-growing bacteria and rely on growth of organisms, MicroResp™ gives more immediate responses to these substrates and reflects activity rather than growth by measuring responses in the first 6 hours. MicroResp™ is currently being used to carry out colorimetric detection or 14C detection measuring CO2 evolved from carbon substrate utilisation in soils. This methodology can be adapted to suit any experimental schedule and microplate reader although calibration is required.


MicroResp Starter Kit (CAT#001)

现货销售

微信请联系

Vicbio微信二维码.jpg


也可通过丁香通商城下单:

https://www.biomart.cn/infosupply/84712339.htm

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说明书请见附件

MicroResp Starter Kit Cat#001 产品明书.pdf

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产品视频

WeChat_20210912230532.mp4


→MicroResp检测原理

http://soils.issas.ac.cn/html/tr/2023/3/tr202207260334.htm#outline_anchor_10


→MicroResp操作视频

https://www.youtube.com/watch?v=IqWOp21My6c


什么是MicroResp™?

MicroResp™是一个独特的基于微孔板的呼吸系统,它使用户能够在一个小且紧凑的空间内分析多达96个土壤、沉积物或水样,

并测试一系列碳源以及进行重复实验。


虽然一些仅针对C-源的测试倾向于选择快速生长的细菌并依赖生物体的生长,但MicroResp™能在前6小时内对这些底物做出更

快速的响应,并通过测量响应来反映活性而非生长。


MicroResp™目前被用于进行比色检测或14C检测,以测量土壤中由碳底物利用产生的CO2。虽然需要校准,但这种方法可以

根据任何实验计划和微孔板读数器进行调整。


MicroResp™主要由三个主要组件组成:

96孔1.2ml深孔板——用于装载碳源和测试材料。

96孔微孔板——包含检测凝胶或在14C检测的情况下,包含用NaOH浸湿的滤纸的96孔等位板。

MicroResp™密封垫——将两块板连接在一起的双向橡胶密封垫。


MicroResp™系统的另外两个必要部分:

夹具——在孵育过程中将三个主要组件固定在一起,确保各孔之间密封良好。

填充装置——帮助用户用固体材料(如土壤)填充深孔板。

虽然MicroResp™是为了在25℃下短时间内(4-6小时)测量CO2的释放而开发的,但它可以根据样品活性和任何温度下的孵育时间进行调整,以模拟自然环境条件。


已证明的应用领域:

土壤健康

毒性测试

农药降解谱

群落水平生理谱(CLPP)

污染诱导的群落耐受性(PICT)

生物修复评价

水生态和毒性


其他潜在应用:

微生物菌株表型分析

动物饲料消化率

种子发芽试验

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MicroResp Starter Kit的主要组件包括:

<<<

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艾美捷科技有限公司

全国服务热线:400-6800-868

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网站:www.amyjet.com

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艾美捷科技代理Epicentre (LGC Biosearch, 原Lucigen)全系列产品。

Epicentre是一家核酸样品制备试剂以及酶的供应商,位于美国威斯康星州麦迪逊的Epicentre生物技术公司成立于1987年,是分子生物学产品的制造商和销售商,其产品广泛应用于RNA扩增及基因表达分析,基于转座子的遗传分析,核酸纯化,DNA序列分析,PCR及RT-PCR扩增,DNA及RNA修饰酶,基因组克隆,体外转录,以及蛋白质研究和纯化等领域。另外,公司拥有广泛的分子生物学产品生产线,可为客户提供蛋白质定制服务。Epicentre品牌现属于LGC集团旗下Lucigen公司。

 

 

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品名规格货号
2'-dNTP (All 4 Mixed)  10 μmole @ 2.5 mM ea.D08104
2'-dNTP (All 4 mixed)  10 μmole @ 25 mM ea.D59104
2´-Fluorine-dCTP (2´-F-dCTP) Solution1 µmolR2F110C
2´-Fluorine-dUTP (2´-F-dUTP) Solution1 µmolR2F110U
Ampligase® DNA Ligase with Buffer  2,500 U @ 100 U/μlA0102K
Ampligase® DNA Ligase without Buffer 10,000 U @ 100 U/μlA0110K
Ampligase® 10X Reaction Buffer  5 mlA1905B
Ampligase® DNA Ligase with Buffer   2,500 U @ 5 U/μlA3202K
Ampligase® DNA Ligase without Buffer  10,000 U @ 5 U/μlA3210K
Ampligase® DNA Ligase with Buffer750 U @ 5 U/μlA32750
Ampligase® DNA Ligase Kit 1,000 U @ 5 U/μlA8101
AmpliScribe™ T7 High Yield Transcription Kit50 rxnsAS3107
AmpliScribe T7-Flash™ Biotin-RNA Transcription Kit10 rxnsASB71110
AmpliScribe T7-Flash Transcription Kit25 RxnsASF3257
AmpliScribe T7-Flash Transcription Kit50 RxnsASF3507
ATP Solution25 µmolRA02825
Baseline-ZERO™ DNase  5000 UDB0715K
Biotin-16-UTP500 nmolBU6105H
CircLigase™ ssDNA Ligase  1000 UCL4111K
CircLigase™ ssDNA Ligase 5000 UCL4115K
CircLigase™ II ssDNA Ligase  1000 UCL9021K
CircLigase™ II ssDNA Ligase  5000 UCL9025K
CopyControl™ Fosmid Autoinduction Solution50 mlAIS107F
CopyControl Fosmid Library Production Kit1 kitCCFOS110
CopyControl HTP Fosmid Library Production Kit1 kitCCFOS059
CopyControl Induction Solution25 mlCCIS125
CopyCutter EPI400 Chemically Competent E.coli 10 × 50 µlC400CH10
CopyCutter EPI400 Electrocompetent E.coli 10 × 50 µlC400EL10
CopyCutter Induction Solution25 mlCIS40025
DuraScribe® T7 Transcription Kit10 RxnsDS010910
DuraScribe T7 Transcription Kit25 RxnsDS010925
End-It DNA™ End-Repair Kit20 RxnsER0720
End-It DNA End-Repair Kit50 RxnsER81050
EpiScript™ RNase H- Reverse Transcriptase10,000 UnitsERT12910K
EpiScript RNase H- Reverse Transcriptase25,000 UnitsERT12925K
Exonuclease I  20,000 UX40520K
Exonuclease VII  250 U @ 10 U/μlEN510250
Exonuclease III  25,000 U @ 200 U/μlEX4425K
Exo-Minus Klenow DNA Polymerase (D355A, E357A)  1,000 U @ 10 U/μlKL11101K
EZ-Tn5™ <DHFR-1> Insertion Kit10 RxnsEZI912D
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EZ-Tn5 <R6Kyori /KAN-2> Tnp Transposome Kit10 RxnsTSM08KR
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FailSafe™ PCR PreMix Selection Kit1 KitFS99060
FailSafe™ PCR System 100 UFS99100
FailSafe™ PCR System250 UFS99250
FailSafe™ Enzyme Mix Only   1000 UFSE5101K
FailSafe™ Enzyme Mix Only   100 UFSE51100
FailSafe™ PCR 2X PreMix A  2.5 mlFSP995A
FailSafe™ PCR 2X PreMix B  2.5 mlFSP995B
FailSafe™ PCR 2X PreMix C 2.5 mlFSP995C
FailSafe™ PCR 2X PreMix D  2.5 mlFSP995D
FailSafe™ PCR 2X PreMix E2.5 mlFSP995E
FailSafe™ PCR 2X PreMix F 2.5 mlFSP995F
FailSafe™ PCR 2X PreMix G  2.5 mlFSP995G
FailSafe™ PCR 2X PreMix H  2.5 mlFSP995H
FailSafe™ PCR 2X PreMix I  2.5 mlFSP995I
FailSafe™ PCR 2X PreMix J  2.5 mlFSP995J
FailSafe™ PCR 2X PreMix K  2.5 mlFSP995K
FailSafe™ PCR 2X PreMix L  2.5 mlFSP995L
Fast-Link™ DNA Ligation Kit100 RxnsLK6201H
Fast-Link DNA Ligation Kit50 RxnsLK0750H
FosmidMAX™ DNA Purification Kit  1 KitFMAX046
Hybridase™ Thermostable RNase H  500 U @ 5 U/μlH39500
Proteinase K  2 mLMPRK092
MasterAmp™ 10X PCR Enhancer  10 PurificationsME81210
MasterAmp™ PCR Optimization Kit  60 templatesMO7201
MasterAmp™ 2X PCR PreMix A 5 mLMO7205A
MasterAmp™ 2X PCR PreMix B 5 mLMO7205B
MasterAmp™ 2X PCR PreMix C 5 mLMO7205C
MasterAmp™ 2X PCR PreMix D 5 mLMO7205D
MasterAmp™ 2X PCR PreMix E 5 mLMO7205E
MasterAmp™ 2X PCR PreMix F 5 mLMO7205F
MasterAmp™ 2X PCR PreMix G 5 mLMO7205G
MasterAmp™ 2X PCR PreMix H 5 mLMO7205H
MasterAmp™ 2X PCR PreMix I 5 mLMO7205I
MasterAmp™ 2X PCR PreMix J 5 mLMO7205J
MasterAmp™ 2X PCR PreMix K 5 mLMO7205K
MasterAmp™ 2X PCR PreMix L 5 mLMO7205L
MasterAmp™ Taq DNA Polymerase  5,000 U @ 5 U/μlQ8205K
MasterAmp™ Taq DNA Polymerase  500 U @ 5 U/μlQ82500
MasterAmp™ Tth DNA Polymerase  250 U @ 5 U/μlTTH72250
MasterPure™ Complete DNA & RNA Purification Kit 200 PurificationsMC85200
MasterPure™ Complete DNA & RNA Purification Kit 10 PurificationsMC89010
MasterPure™ DNA Purification Kit for Blood, Version II 400 mL of Whole BloodMB711400
MasterPure™ Gram Positive DNA Purification Kit  100 PurificationsMGP04100
MasterPure™ Yeast RNA Purification Kit  100 PurificationsMPY03100
MasterPure™ Yeast DNA Purification Kit  200 PurificationsMPY80200
MaxPlax™ Lambda Packaging Extract10 ExtMP5110
MaxPlax Lambda Packaging Extract20 ExtMP5120
MaxPlax Lambda Packaging Extract 5 ExtMP5105
MMLV High Performance Reverse Transcriptase25,000 URT80125K
MMLV Reverse Transcriptase 1st Strand cDNA Synthesis Kit50 RxnsMM070150
MPC Protein Precipitation Solution     500 mLMMP095H
NTPs: ATP, CTP, GTP, UTP Solutions25 umol @100mM eachRN02825
OmniCleave™ Endonuclease  50,000 UOC7850K
Plasmid-Safe™ ATP-Dependent DNase  1,000 U @ 10 U/μlE3101K
Plasmid-Safe™ ATP-Dependent DNase  10,000 U @ 10 U/μlE3110K
Poly(A) Polymerase Tailing Kit  50 rxnsPAP5104H
QuickExtract™ DNA Extraction Solution 1.0   50 mLQE09050
QuickExtract™ DNA Extraction Solution 1.0   5 mLQE0905T
QuickExtract™ FFPE DNA Extraction Kit  50 mLQEF81050
QuickExtract™ Plant DNA Extraction Solution  50 mLQEP70750
QuickExtract™ RNA Extraction Kit 50 mLQER090150
Ready-Lyse™ Lysozyme Solution 4000000R1804M
Ready-Lyse™ Lysozyme Solution 10000000R1810M
Rec J Exonuclease  250 U @ 10 U/μl RJ411250
Red Cell Lysis Solution     1200 mLMRC0912H
RiboGuard™ RNase Inhibitor10,000 URG90910K
RiboGuard RNase Inhibitor2,500 URG90925
Ribonuclease R (RNase R)  250RNR07250
RNA 5' Polyphosphatase 200RP8092H
RNase A   2 mLMRNA092
RNase I  1000 UN6901K
RNase-Free DNase I  5,000 U @ 1 U/μlD9905K
RNase-Free DNase I  10,000 U @ 1 U/μl  D9910K
Sterile Nuclease-Free Water   50 mLW7350ML
T4 Polynucleotide Kinase, Cloned  3,000 UP0503K
T4 RNA Ligase 2, Deletion Mutant  10,000 U @ 200 U/ulLR2D11310K
T4 RNA Ligase 2, Deletion Mutant  2,000 U @ 200 U/ulLR2D1132K
T7 R&DNA™ Polymerase  5,000 U @ 50 U/μlD7P9205K
T7 RNA Polymerase  50,000 U @ 1,000 U/μlTH950K
TE Buffer      70 mLMTE0970
Terminator™ 5'-Phosphate-Dependent Exonuclease  40 U @ 1 U/μlTER51020
Tissue & Cell Lysis Solution  600 mLMTC096H
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TransforMax EC100 Electrocompetent E.coli10 × 100 uLEC10010
TransforMax EC100D pir+ Electrocompetent E. coli,5 × 100 uLECP09500
TransforMax EC100D pir-116 Electrocompetent E. coli,5 × 100 uLEC6P095H
TransforMax EPI300 Chemically Competent E.coli10 × 50 uLC300C105
TransforMax EPI300 Electrocompetent E.coli10 × 100 uLEC300110
TransforMax EPI300 Electrocompetent E.coli5 × EC300 110EC300150
Transformax EPI300-T1R Electrocompetent E.coli10 × 100 uLEC02T110
TypeOne™ Restriction Inhibitor 100 µgTY0261H
Uracil N-Glycosylase 1,000 UUG131K
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